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   DIVISION OF EXPERIMENTAL PATHOPHYSIOLOGY & IMMUNOLOGY
   Laboratory of Biophysics/Biooptics

     Guenther Boeck, Ph.D.
Telephone: +43 (0) 512-9003.70985

Scientific interests

The aim of our biophysics-immunofluorescence technology group lies in the development of new fluorescence techniques for cell biology that are required within the framework of most other projects within the Institute of Pathophysiology. This includes flow cytometry and confocal microscopy. Some examples of these developments are the following:

a)   the use of a fluorescence-activated cell sorter (FACS) for receptor demonstration and biochemical characterization on a single
cell level

b)   demonstration of apoptosis with various staining methods

c)   detection of apoptosis together with Ca2+ flux together with Ca2+ flux

d)   image analysis and 3D reconstruction of single cells

Selected References

G.BOECK, M.HILCHENBACH, K.SCHAUENSTEIN and G.WICK
Photometric analysis of antifading reagents for immunofluorescence with laser and convential illumination sources.
J.Histochem.Cytochem. 33:699-705 (1985)

K.N.TRAILL, G.BOECK, U.WINTER, M.HILCHENBACH, G.JUERGENS and G.WICK
Simple method for comparing large numbers of flow cytometry histograms exemplified by analysis of the (CD4) T4 antigen and LDL receptor on human peripheral blood lymphocytes.
J. Histochem.Cytochem. 34:1217-1221 (1986)

G.WICK, H.HENGSTER, G.BOECK and M.P.DIERICH
Improvement of immunofluorescence for the diagnosis of AIDS using laser microscopy.
J.Histochem.Cytochem. 36:793-796 (1988)

G.BOECK, L.A.HUBER, G.WICK and K.N.TRAILL
Use of a FACS III for fluorescence depolarization with DPH.
J.Histochem.Cytochem.37:1653-1658 (1989)

R.SGONC, G.BOECK, H.DIETRICH, J.GRUBER, H.RECHEIS and G.WICK
Simultaneous determination of cell surface antigens and apoptosis.
Trends in Genetics 10: 41-42 (1994)

G.BOECK, P.STEINLEIN and L.A.HUBER
Cell biologists sort things out: analysis and purification of intracellular organelles by flow cytometry.
Trends Cell Biol. 7: 499-503 (1997)

C.RIESER, G.BOECK, H.KLOCKER, G.BARTSCH and M.THURNHER
Prostaglandin E2 und tumor necrosis factor a cooperate to activate human dendritic cells: synergistic activation of interleukin 12 production.
J.Exp.Med.186: 1603-1608 (1997)

G.BOECK, P.STEINLEIN, M.HABERFELLNER, J.GRUENBERG and L.A.HUBER
Fluorescence-activated sorting of endocytic organelles.
in: Cell Biology. A Laboratory Handbook, Academic Press 2: 63-69 (1998)

Y.HU, G.BOECK, G.WICK and Q.XU
Activation of PDGF receptor a in vascular smooth muscle cells by mechanical stress.
FASEB J. 12:1135-1142 (1998)

R.SGONC, Ch.FUERHAPTER, G.BOECK, R.A.SWERLICK, P.FRITSCH and N.SEPP
Induction of apoptotis in human dermal microvascular endothelial cells and infantile hemangiomas by interferon-alpha
Int.Arch.Allergy Immunol. 117: 209-214 (1998)

I.FIALKA, P.STEINLEIN, H.AHORN, G.BÖCK, P.D.BURBELO, M.HABERFELLNER, F.LOTTSPEICH, K.PAIHA, C.PASQUALI, L.A.HUBER
Identification of syntenin as a protein of the apical early endocytic compartment in madin-darby canine kidney cells.
J.Biol.Chem. 274: 26233-26239 (1999)

N.SPOETTL, B.WIRLEITNER, G.BÖCK, B.WIDNER, D.FUCHS and G.BAIER-BITTERLICH
Reduced Pteridine derivates induce apoptosis in human neuronal NT2/HNT cells.
Immunobiology 201: 478-491 (1999/2000)

G.BOECK
Current status of flow cytometry in cell and molecular biology.

in: International Review of Cytology - K.W.Jeon (ed.), Acacemic Press 204: p. 239-298 (2001)

C.ENZINGER, B.WIRLEITNER, C.LUTZ, G.BOECK, B.TOMASELLI, G.BAIER, D.FUCHS and G.BAIER-BITTERLICH
7,8-Dihydroneopterin induces apoptosis of Jurkat T-lymphocytes via a Bcl-2-sensitive pathway.
Eur.J.Cell.Biol. 81: 197-202 (2002)

B.WIRLEITNER, D.REIDER, S.EBNER, G.BOECK, B.WIDNER, M.JÄGER, H.SCHENNACH, N.ROMANI and D.FUCHS
Monocyte-derived dendritic cells release neopterin.
J.Leukocyte Biol. 72: 1148-1153 (2002)

M.Saurwein-Teissl, T.L.Lung, F.Marx, C.Gschoesser, E.Asch, I.Blasko, W.Parson, G.Böck, D.Schoenitzer, E.Trannoy, B.Grubeck-Loebenstein
Lack of antibody production following immunization in old age: association with CD8+CD28- T cell clonal expansions and an imbalance in the production of Th1 and Th2 cytokines.
J. Immunol. 168(11): 5893-5899 (2002)

B.WIRLEITNER, G.OBERMOSER, G.BÖCK, G.NEURAUTER, H.SCHENNACH, N.SEPP and D.FUCHS
Induction of apoptosis in human blood T cells by 7,8-dihydroneopterin: the difference between healthy controls and patients with systemic lupus erythematosus
Clin.Immunol. 107: 152-159 (2003)

A.VILLUNGER, E.M.MICHALAK, L.COULTAS, F.MUELLAUER, G.BOECK, M.J.AUSSERLECHNER, J.M.ADAMS, A.STRASSER
p53- and drug-induced apoptotic responses mediated by BH3-only proteins puma and noxa
Science 302: 1036-1038 (2003)

M.J.AUSSERLECHNER, P.OBEXER, G.BOECK, S.GELEY and R.KOFLER
Cyclin D3 and c-myc control glucocorticoid-induced cell cycle arrest but not apoptosis in lymphoblastic leukemia cells.
Cell Death Differ. Oct. 24 (2003)


   
   
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