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INSTITUTE OF GENERAL & EXPERIMENTAL PATHOLOGY
Leonardo da Vinci Programme
Immunofluorescence and Immunohistochemistry Training Course Innsbruck, Austria February 7 - 11, 2000
Organizers: Prof. Dr. Georg WICK, Institute for General and Experimental Pathology, University of Innsbruck, Medical School Fritz-Pregl-Strasse 3, A-6020 Innsbruck, Tel.: 0043/512/507 3100, Fax: 0043/512/507 2867, E-mail: exper-pathologie@uibk.ac.at Dr. Heinz GUNDLACH, Carl Zeiss, D-73446 Oberkochen, Tel.: 0049/7364/20 2071, Fax: 0049/7364/20 4013, E-mail: gundlach@zeiss.de Location: Medizinisch-Theoretische Institute, Hörsaal 3/Hörsaal 4, University of Innsbruck, Medical School, Fritz-Pregl-Strasse 3 A-6020 Innsbruck Participants: Medical and Ph.D. students as well as postdocs interested in basic aspects of light microscopy and practical applications of immunofluorescence and immunohistochemistry Application: Send informal letter stating professional experience and reasons for application as well as C.V. to Dr. G. Wick at the above mentioned address. Restricted attendance! Fee: ATS 4.000,-- (accommodation not included), Transfer money to Bank für Tirol und Vorarlberg, Innsbruck, account no. 100-167999, or send cheque to Dr. G. Wick made payable to Leonardo da Vinci Training Course Lecturers: Georg WICK Günther BÖCK Harald NIEDEREGGER Siegfried SCHWARZ Roswitha SGONC Jan WIEGERS Institute for General and Experimental Pathology, University of Innsbruck, Medical School, Fritz-Pregl-Strasse 3, A-6020 Innsbruck Heinz GUNDLACH Carl Zeiss, D-73446 Oberkochen Wolf MALKUSCH Carl Zeiss Vision, Zeppelinstrasse 4, D-85399 Hallbergmoos Technical assistance: Pia MÜLLER Ernst RAINER Institute for General and Experimental Pathology, University of Innsbruck, Medical School, Fritz-Pregl-Strasse 3, A-6020 Innsbruck Technical support and equipment: CARL ZEISS, D-73446 Oberkochen ZEISS Österreich GmbH, Modecenterstrasse 16, A-1034 Wien HAMAMATSU Photonics Deutschland GmbH, Arzbergstrasse 10, D-82211 Herrsching MICROM GmbH, Robert-Bosch-Strasse 49, D-69190 Walldorf Administration: Andrea HOHENAUER Institute for General and Experimental Pathology, University of Innsbruck, Medical School, Fritz-Pregl-Strasse 3, A-6020 Innsbruck Programme Monday, February 7, 2000: 09.00 - 12.00: Introduction to immunology, part I (G. Wick) 14.00 - 18.00: Introduction to immunology, part II (G. Wick) Introduction to light microscopy (H. Gundlach) Theory of image formation in the microscope (H. Gundlach) Tuesday, February 8, 2000: 09.00 - 12.00: Principles of immunofluorescence and immunohistology - Main immunofluorescence and immunohistology techniques (G. Wick) - Fluorescence microscopy (H. Gundlach) - Molecular Modelling of Immunological Molecules (S.Schwarz) - Optical contrast enhancement techniques (H. Gundlach) - Microphotography (principles, instrumentation, films, filters) (H. Gundlach) 14.00 - 17.00: Practical exercises - Direct and indirect immunofluorescence tests - Triple staining techniques Wednesday, February 9, 2000: 09.00 - 12.00: - Standardization of immunofluorescence and immunohistochemical methods (G. Wick) - Fixation methods (G. Wick) - Immunohistochemistry - methods on paraffin and frozen sections (G. Wick) 14.00 - 17.00: Practical exercises - Preparation of frozen sections - Double staining techniques - Immunohistochemistry on frozen sections Thursday, February 10, 2000: 09.00 - 12.00: - Principles of tissue and organ culture (J. Wiegers) - Cytometry (principles and quantitative applications) (G. Böck) 14.00 - 17.00: Practical exercises - Membrane immunofluorescence 17.15 - 18.00: Invited guest lecture Friday, February 11, 2000: 09.00 - 12.00: - Confocal laser microscopy (H. Gundlach, H. Niederegger) - Fluorescence digital imaging microscopy (H. Gundlach) - Image processing and image analysis (W. Malkusch) - Discussion of scientific and methodological problems between participants and faculty 14.00 - 17.00: Practical exercises - Fluorescence activated cell sorting with interpretation of results - Demonstration of confocal laser scanning microscopy and 3D microscopy - Video microscopy 20.00: Farewell party Saturday & Sunday, February 12 & 13, 2000: Facultative skiing weekend with those participants who would like to stay on for two additional days.
If you have questions, please, contact Ms. Barbara Gschirr, secretary of the Institute, using the above adress, phone or fax numbers, respectively or by email.
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