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Instrumentation

N-Terminal and Internal Protein Sequencing

ABI Procise Model 492 Edman Micro Sequencer

 

Capillary Electrophoresis:

Beckman Instruments PA800 and CESI8000 Electrophoresis systems

ESI-MS:

Orbitrap Eclipse, Orbitrap Eclipse with FAIMS Pro and Q Exactive HF (ThermoScientific)

HPLC 

Various analytical and semipreparative instrument systems e.g. HP 1100 System with UV-Visible or Fluorescence detector (Agilent Technologies). Vanquish Neo, UltiMate 3000 systems XRS for micro-HPLC and RSLCnano system (ThermoScientific) for nano-HPLC separations controlled through Chromeleon software.

Atomic Absorption Spectrometry (AAS):

Solaar M6 Dual Zeeman spectrometer (ThermoScientific) and Microwave Digestion system (CEM)

 

Software

Proteome Discoverer 3.1 with Chimerys Lite (Thermo Scientific)

XLinkX for PD 3.1

Mascot (Matrix Science)

Protein Pilot (Sciex)

MaxQuant  (Max Planck Institute of Biochemistry)

StavroX & MeroX   

Skyline

DIA-NN